Enzymology Lab Report Example | Topics and Well Written Essays - 1250 words

Enzymology - Lab narration ExampleSubstrate concentration is directly proportional to action rate up to a peak beyond which the reaction rate slows down and settles at a constant level because of the enzymes bound factor (Yada, 2004 Whitehurst & Oort, 2009). Temperature and pH, however, increase the activity rate up to optimum levels beyond which the activity rate reduces. The optimum temperature and pH level for most enzymatic activity is room temperature and the pH level of seven (Rastogi, 2010). Enzyme inhibitors ar another set of factors that reduce enzymes activity rate (Seager & Slabaugh, 2010).This report investigates the effects of substrate concentration, pH level, temperature and inhibitor hydrogen peroxide on bovine liver-coloreds activity rate. It seeks to answer the question how does the factors affect activity rate of Bovine liver?Care was taken to ensure that the atomic number 8 gas sensor was out of use(p) into the USB interface to launch the PAS portal windo w. This was followed by a click on the DataStudio icon to launch the program. The fabricate experiment button was then clicked to generate a Digit box. A click on the apparatus button and a change of oxygens measurement unit to ppm then followed before calibration of the sensor. Amounts of liver extract were then incubated at given temperatures for five minutes before addition of substrate and push on the oxygen sensor stopper. With the stopper firmly held, the substrate was mixed and incubated while noting the make out of released oxygen. The experiment was repeated for different temperatures.With the set ups at the room temperature, tests were conducted at the pH levels of two, four, six, seven, eight, ten, and twelve respectively. In each set up and for every pH level, 6 ml of buffer, 3 ml of liver extract and 4 percent of H2O2 were mixed in that consecutive order. This was followed by measurement of the level of generated oxygen for calculating reaction rates at each pH leve l.With the